Description
This kit is a sandwich enzyme immunoassay for the direct determination of endostatin in mouse/rat serum and plasma samples. In a first step, 1+50 pre-diluted STD/sample/CTRL and conjugate (goat anti-endostatin-HRPO) are pipetted into the wells of the microtiter strips, which are pre-coated with anti mouse endostatin antibody. Endostatin present in the STD/sample/CTRL binds to the pre-coated antibody in the well and forms a sandwich with the detection antibody in the conjugate. In the washing step all non-specific unbound material is removed. In a second step, the substrate (TMB Tetramethylbenzidine) is pipetted into the wells. The enzyme catalysed colour change of the substrate is directly proportional to the amount of endostatin. This colour change is detectable with a standard microtiter plate ELISA reader. A dose response curve of the absorbance (optical density, OD at 450 nm) vs. standard concentration is generated, using the values obtained from the standard. The concentration of mouse/rat endostatin in the sample is determined directly from the dose response curve